Journal: Frontiers in Immunology
Article Title: Exploring the role of neutrophils in inflammatory pain hypersensitivity via single-cell transcriptome profiling
doi: 10.3389/fimmu.2025.1552993
Figure Lengend Snippet: Regulation of S100b and CaMKIIβ protein expression by NAMO. (A, B) Western blot analysis was used to measure the protein expression levels of S100b and CaMKIIβ in peripheral blood, plantar tissue, and Ly6G + cells from mice that underwent surgery and CFA treatment, both before and after NAMO administration. (C, D) Immunohistochemical analysis was used to assess S100b protein expression in the plantar tissues of surgically treated and CFA-treated mice both before and after NAMO treatment. Cells with positive staining are indicated by a dark brown color (scale bar = 100 μm).
Article Snippet: Immunohistochemical (IHC) analysis was performed with S100b rabbit anti-mouse polyclonal IgG (1:1500; Proteintech Group, Inc., Chicago, USA) and horseradish peroxidase-conjugated goat anti-rabbit IgG (1:200; Servicebio, Hubei, China).
Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining